Clonexpress multis one-step cloning kit
WebProduct Description. ClonExpress MultiS One Step Cloning Kit (Vazyme, #C113) is a new version cloning kit based on ClonExpress One Step Cloning technology. Exnase … The Mut Express MultiS Fast Mutagenesis Kit V2 is designed for rapid mutagene… The Mut Express II Fast Mutagenesis Kit V2 is designed for rapid site-directed m… ClonExpress UItra One Step Cloning kit (Vazyme, #C115) is a novel cloning kit, a… WebFeb 24, 2024 · Primer synthesis and Sanger sequencing were performed by GENEWIZ (Suzhou, China). All enzymes were obtained from Vazyme (Nanjing, China) or TransGen (Beijing, China). Unless otherwise stated, plasmids were constructed via recombination by using the ClonExpress MultiS One Step Cloning Kit (Vazyme, Nanjing, China).
Clonexpress multis one-step cloning kit
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WebThis product is the third generation TOPO cloning kit, which contains Topoisomerase, vector and self-ligation inhibitor. The kit uses a new type of Topoisomerase, with the optimal buffer, the enzyme activity is higher, and the high-efficiency ligation can be completed in 5 minutes. ... ClonExpress MultiS One Step Cloning Kit C113. USD$ 96.00 ... WebProduct Introduction. Mut Express® II Fast Mutagenesis Kit V2 is a site-directed mutagenesis system based on ClonExpress® rapid cloning technology. Using this kit, the target plasmid amplification product is digested with DpnI, ClonExpress® recombination and circularized, and then directly transformed to complete the site-directed mutation.
WebMar 1, 2024 · Step Cloning Kit (Vazyme, Nanjing, China), while the hygB expression cassette was assembled from P gpdA , hygB , and trpC by ClonExpress MultiS One Step Cloning Kit. WebAug 21, 2024 · The assembly of multi-fragments was achieved by ClonExpress™ II One Step Cloning Kit or ClonExpress MultiS One Step Cloning Kit (Vazyme Biotech Co., Ltd., China). Plasmid DNA and yeast genomic DNA were obtained using TIANprep Rapid Mini Plasmid Kit (TIANGEN) and TIANamp Yeast DNA Kit (TIANGEN), respectively. …
Webvitro by using ClonExpress MultiS One-Step Cloning Kit, and the plasmid backbones were obtained via PCR. The plasmid pPIC9K backbone was linearized by Sal I, and the transformant resistant to 0.25 mg/mL geneticin was selected from the His+ transformants. When the plasmid pPICZαA backbone was used, the linearized plasmid was WebFeb 17, 2024 · PD7:SgSQE1:Thsp and 35S:SgCS:Tnos were inserted at the EcoRI/HindIII restriction sites of a pCAMBIA1300 binary plasmid via a ClonExpress MultiS One Step Cloning Kit. Similarly, UBQ:SgUGT269-1:Tmas ...
WebClonExpress® technology is a simple, fast and efficient DNA seamless cloning technology, which can clone the insert into any position of any vector. Use any method to linearize …
WebClonExpress ® technology is a simple, fast and efficient DNA seamless cloning technology, which can clone the insert into any position of any vector. Linearize the … hobby lobby deerfield beach phone numberWebJan 22, 2024 · In particular, genes encoding ent-copalyl diphosphate synthase 1 (CPS1), which catalyzes the first committed step in GA biosynthesis, and GA3 oxidase 1 (GA3ox1), ... Shanghai, China) and then cloned into the pENTR vector by recombination reaction (ClonExpress MultiS One Step Cloning Kit, C113-02; Vazyme, Nanjing, China). These … hsbc soc reportWebMay 19, 2024 · ClonExpress ® II One Step Cloning Kit and ClonExpress ® MultiS One Step Cloning Kit or equivalent used for isothermal assembly. 9. Genomic DNA purification kit. 10. Plasmid DNA purification kit. 11. PCR cleanup kit. 12. DNA gel-extraction kit. 13. 0.4 mm electroporation cuvettes. hobby lobby definition of hanger kitchen signWebThe cloning products can then be directly transformed to competent cells with apositive rate >95%. ClonExpress MultiS One Step Cloning Kit is a new version cloning kit based … hobby lobby deer head decorWeb作为新一代重组克隆试剂盒，ClonExpress® MultiS 专门针对多片段重组反应进行了优化，可实现一次最多 5 个插入片段的连续拼接克隆。. 试剂盒独特的非连接酶依赖系统，大 … hsbc soft footprint dipWebThe PCR products contain A at the 3’-end and can be directly cloned into T-Vectors, compatible with ClonExpress II One Step Cloning Kit series (Vazyme, #C112, #C113). Product Manual (pdf) ... ClonExpress MultiS One Step Cloning Kit (Vazyme, #C113) Mut Express II Fast Mutagenesis Kit V2 (Vazyme, #C214) hsbc software company in puneWebMar 14, 2024 · ClonExpress ® technology is a fast, simple and efficient DNA seamless cloning technology, enabling the insert of any position of any vector to be cloned. The … hobby lobby delish boutique yarn