WebTwo-hybrid screens using CPRF1 and CPRF2 as "baits" resulted in the isolation of four novel parsley proteins which interact with either CPRF1 or CPRF2 in vivo. Three of these factors represent new parsley bZIP factors, designated CPRF5-CPRF7, whereas the fourth, named CPRF1-interacting protein (CIP), shows no homology to any other known protein. CPRF1 specifically binds an ACGT-containing element which is located within the composite regulatory unit that is necessary and sufficient for light activation of the parsley chalcone synthase (CHS) minimal promoter. Expression studies at the mRNA level demonstrated thatCPRF1 mRNA is present in all organs of light-grown plants in whichCHS mRNA ...

Functional analysis of a light-responsive plant bZIP transcriptional ...

WebCPRF1 is listed in the World's largest and most authoritative dictionary database of abbreviations and acronyms CPRF1 - What does CPRF1 stand for? The Free Dictionary WebJun 1, 1998 · Further studies have indicated that CPRF1 contributes to the regulation of chs and that of the cprf1 gene itself. A second promoter element, previously identified by in vivo footprinting, has affinity to the MYB305 protein and to a novel MYB protein from parsley, MYB1 (Ref. [39]). openseas nft size

Light-regulated transcriptional networks in higher plants

WebFeb 12, 2002 · Although a few examples of strong and rapid gene silencing by elicitor have been observed (7, 8, 18), the responsible promoter elements have not been identified.Among the silenced genes are those encoding ACO and chalcone synthase (CHS) (), catalyzing, respectively, a major supply reaction and the first committed step of UV … WebHowever, CPRF1 levels increase only transiently after light treatment, and transcription might be blocked by the binding of CPRF1 to its own promoter 17. Such a 'gas-and-brake' mechanism is widely ... WebCPRF1 genomic DNA was cloned and the gene was sequenced. Analysis of the sequence data revealed the existence of 12 exons and 11 introns within a stretch of about 9 kb. A second RNA species hybridising to CPRF1 probes was identified as an alternatively spliced, additional CPRF1 transcript containing intron 8. open season 1080p