WebInstruction manual 1 * Special pack delivered on specific customer order. Description Medium properties HisTrap HP 1 ml and 5 ml columns are packed with 1 ml and 5 ml of … WebHiTrap™ NHS-activated HP Columns 至適条件のpH 8.3では、 ε-アミノ基（リジン残基側鎖）はα-アミノ基（N末端アミノ酸）よりも優先的に反応し、15～30分で反応が完了します。 反応基のカルボキシル基はN-hydroxysuccinimide（NHS）により活性エステル化されており、リガンド溶液を注入するだけでリガンドの1級アミノ基（-NH 2 ）とアミド結合を形 …

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WebBuffer/sample composition is not optimal. Check pH and composition of sample and binding buffer. Ensure that the concentration of chelating or strong reducing agents in the sample … HisTrap HP 1 ml and 5 ml columns are prepacked with Ni Sepharose High Performance , which consists of 34 μm highly cross-linked agarose beads with an immobilized chelating group. The medium has then been charged with Ni2+-ions. Several amino acids, for example histidine, form complexes with many metal ions. Ni Sepharose High Performance csi s2 e11

HisTrap HP, 1 x 5 ml Cytiva

WebHisTrap™ HP His-tagタンパク質精製用カラム このページを印刷 ご注文情報 詳細情報 関連製品 高い結合容量（40 mg/ml）* さまざまな可溶化剤（DTT、メルカプトエタノール、ureaなど）存在下で利用可能 短時間精製、システムまたはポンプ接続可能 金属イオン（Ni 2+ ）が強固に結合されており金属イオン漏れがほとんどなし * Dynamic Binding … Web71714900 AS 5 Table 2. HiTrap SP HP and HiTrap Q HP columns characteristics 1 Median particle size of the cumulative volume distribution. 2 Dynamic binding capacity measured … Web17 dic 2024 · After centrifugation, the supernatant was loaded in a HisTrap HP column. AcrF proteins were eluted with a stepwise gradient of 1.0 M imidazole, digested with TEV protease overnight for removal of 6XHis and SUMO-tags, and purified with ion-exchange chromatography using either a Heparin HiTrap Q HP or Heparin HiTrap SP HP … csi s2 e1 cast