Mwasabi fluorescent antibody
WebFeb 21, 2024 · A natural monomeric infrared fluorescent protein (IFP), mIFP, was designed in 2015 [ 40 ]. mIFP has been proven to have sound imaging effects in Drosophila larvae and neurons. Shcherbakova et al. designed three bright monomeric NIR FPs with distinct spectra, namely, miRFP670, miRFP703, and miRFP709 [ 41 ]. WebAlleleustrious Recombinant mWasabi [ [100µg,ABP-FP-WPNCSPR]] (100μg) Expressed from E.coli; with a 6xHis tag at the N-terminus Recombinant mWasabi-GFP // $199.00 Choose Options
Mwasabi fluorescent antibody
Did you know?
WebOct 23, 2014 · Tandem fluorescent protein-tagged LC3s that were comprised of a protein tag that emits green fluorescence (e.g., EGFP or mWasabi) fused with another tag that emits red fluorescence (e.g. mCherry or TagRFP) were used for monitoring the maturation step of mammalian autophagosomes. WebIn vitro, mWasabi is 1.6-fold brighter than EGFP and is photostable. It also has narrow excitation and emission peaks (max excitation at 493 nm and max emission at 509 nm), making it useful in multicolor labeling …
WebThe green fluorescent proteins, EGFP (A–D), mWasabi (E–I), and pHluorin (J–P), were expressed in the Huh7.5.1 cells. After fixation and permeabilization of the cells, the cells were buffered at... WebTherefore, it is desirable to choose a monomeric green fluorescent protein that is more acid sensitive than EGFP in the assay of autophagic flux. Here, we report on an mTagRFP-mWasabi-LC3 reporter, in which mWasabi is more acid sensitive than EGFP and has no fluorescence in acidic lysosomes.
WebPlasmid L5 attB::Pleft* mWasabi was a gift from Clifton Barry III (Addgene plasmid # 169409 ; http://n2t.net/addgene:169409 ; RRID:Addgene_169409) For your References section: Development and Optimization of Chromosomally-Integrated Fluorescent Mycobacterium tuberculosis Reporter Constructs. WebPlasmid mWasabi-Mito-7 from Dr. Michael Davidson's lab contains the insert Mito and is published in Cold Spring Harb Protoc. 2009 Dec;2009(12):pdb.top63. doi: 10.1101/pdb.top63. ... Viral Preps and Antibodies. What is virus associated DNA, and why do I have to order it? What virus titer will I receive? ... Fluorescent protein tracking and ...
WebPlasmid mWasabi-N1 from Dr. Michael Davidson's lab is published in BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/1741-7007-6-13. ... Viral Preps and Antibodies. What is virus associated DNA, and why do I have to order it? What virus titer will I receive? ... Hue-shifted monomeric variants of Clavularia cyan fluorescent protein: ...
WebLearn more about Addgene materials from user-contributed reports describing AAV and antibody experiments. Sequence Analyzer. Basic analysis for a user-entered sequence; includes restriction sites and map. Vector Database. Digital collection of empty plasmid backbones from publications and commercially available sources gothic fiction is an example of a literaryWebThe pKa of mWasabi is 6.5 [131], thus compared to EGFP, its fluorescence decreases at acidic environments more sensitively. In addition, mTagRFP (pKa = 3.8) is very stable in acidic pH [17,132].... gothic fiction factsWebSnapGene Viewer. SnapGene Viewer is free software that allows molecular biologists to create, browse, and share richly annotated sequence files. Gain unparalleled visibility of … child adirondack chair wood foldingWebmWasabi = mTFP1 + A66S-K139E-H163M-S216I. Excitation = 493; Emission = 509 How to cite this plasmid ( Back to top ) These plasmids were created by your colleagues. Please … child adjustment inventoryWebDec 9, 2024 · mWasabi dN was introduced downstream between NcoI and HindIII sites, using primers 39 and 40. In order to construct the P s myc 1806 and P left 1806 fusion products, we applied Gibson Assembly. As vectors, pL5 containing either P s myc-mWasabi dN or P left *-HismClover3 were used. The plasmid backbone was amplified with primers … child ad litemWebLearn more about Addgene materials from user-contributed reports describing AAV and antibody experiments. Sequence Analyzer. Basic analysis for a user-entered sequence; includes restriction sites and map. Vector Database. ... fluorescent protein joins mTFP1 and … gothic fiction outgrowthWebNational Center for Biotechnology Information child adjustable height desk